Which of the following is NOT a characteristic of a vacuole?

The characteristic that is identified as not belonging to a vacuole is that it contains nuclei. Vacuoles are membrane-bound sacs found in plant and fungal cells, primarily serving roles in storage, waste disposal, and maintaining turgor pressure. They are typically filled with fluid, which helps maintain the shape and structure of the cell. Additionally, vacuoles can occupy a significant portion of the cell volume, particularly in mature plant cells, aiding in cellular processes and overall stability.

In contrast, nuclei are distinct organelles that house the cell's genetic material and are responsible for regulating growth and reproduction. They are separate from vacuoles and perform different functions within the cell. Therefore, the presence of nuclei within a vacuole is inaccurate and does not align with the actual characteristics of vacuoles.